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Corning Life Sciences
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Image Search Results
Journal: Experimental cell research
Article Title: Matrix metalloproteinase 9 inhibits the motility of highly aggressive HSC-3 oral squamous cell carcinoma cells.
doi: 10.1016/j.yexcr.2019.01.018
Figure Lengend Snippet: Fig. 4. (A) The amount of fibronectin in the control and shMMP9 HSC-3 cell homogenates (20 µg of soluble protein) was analysed with Western blotting. (B) Migration of the shMMP9 and control HSC-3 cells was analysed with a scratch wound assay on uncoated (empty) and 10 µg/ml fibronectin (Fn)-coated size 24 wells. The wounds were photographed with an EVOS photo microscope at 8 h and 24 h after scratching. The area of the open wound was measured with Fiji software and the results are presented as a percentage of wound closure (n = 4 scratch wounds analysed per condition). P-values were calculated using Student's T-test. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: The membranes were blocked with 5% milk powder or 5% BSA (for Phospho-antibodies) in Tris-buffered saline 0.1% Tween 20 and incubated overnight with p44/42 MAPK (Erk1/2), Phospho-p44/ 42 MAPK (Erk1/2) (Thr202/Tyr204), Akt, Phospho-Akt (Ser473) (1:1000, all from Cell Signaling Technology),
Techniques: Control, Western Blot, Migration, Scratch Wound Assay Assay, Microscopy, Software